所有项目
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摘要:
Ultrasound mediated microbubble cavitation holds great potential for non-invasive and targeted drug delivery. However, the interplay between acoustic parameters, bubble dynamics, and resulting cellular responses remains unclear, hindering the safety improvement and optimization of the technique. This study examined the effects of ultrasound pulse sequences on microbubble dynamics and bioeffects...
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Hyperosmotic stress triggers cellular dehydration and macromolecular crowding, leading to rapid biomolecular condensation. However, the individual roles of dehydration and crowding have been unclear, and the design principles of osmosensors remain poorly understood. Here, we identify a conserved sensor module comprising an osmo-sensing α-helix adjacent to an intrinsically disordered region (IDR)....
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摘要:
The insulin receptor (IR) is central to the regulation of glucose and lipid metabolism. Although insulin is its primary ligand, insulin-like growth factors I and II (IGF-I and IGF-II) also engage IR, albeit with reduced affinity. The structural basis of cooperative ligand binding, however, has remained poorly understood. Here, we report cryo-Electron Microscopy (cryo-EM) structures of IR in...
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摘要:
Chromosome substitution strains (CSS) are critical tools for dissecting complex traits, although iterative breeding steps and intraspecific compatibility requirements limit conventional approaches. Here, we developed an optimized TEAM platform for chromosome replacement combing CRISPR/Cas9–mediated chromosome elimination with microcell-mediated chromosome transfer (MMCT). Using this approach, we...
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摘要:
Obesity-associated metabolic inflammation is driven by lipid-laden macrophages that release proinflammatory cytokines such as interleukin-1β, yet how macrophages sense lipid overload to trigger inflammation remains unclear. Here, we identify sphingomyelin sensing as a key mechanism linking lipid metabolism to inflammatory activation. We show that macrophages detect specific sphingomyelin species...
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摘要:
Respiratory syncytial virus (RSV) poses a significant global health challenge, particularly affecting infants, the elderly, and immune compromised individuals. Despite recent progress in the development of vaccines and monoclonal antibodies, effective antiviral therapies remain limited. To advance the discovery of antiviral drugs, we have developed a dual-reporter RSV minigenome system, providing...
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摘要:
The mitochondrial unfolded protein response (UPRmt) is a critical mechanism for restoring cellular homeostasis and promoting longevity upon mitochondrial stress. However, the mechanisms that sense specific mitochondrial damage-associated signals and activate UPRmt remain largely unknown. Through a genome-wide RNAi screen in Caenorhabditis elegans, we identified the AT-hook protein LIN-15B as a...
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Super-resolution imaging with RhoBAST fluorogenic RNA aptamer sheds new light on decoding RNA dynamics with high spatiotemporal resolution. To uncover the molecular basis underlying its exceptional photophysical properites, we determined the first free-form structure of RhoBAST, along with its ligand-bound complexes with TMR-DN and related analogs. RhoBAST adopts an inverted 'V'-shaped...
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摘要: Objective: Noninvasive prenatal testing (NIPT) based on cell-free DNA (cfDNA) analysis is widely used for detecting fetal aneuploidies globally, such as Trisomy 21 (T21) testing. Despite its high sensitivity, current NIPT methods have a non-negligible rate of false-negative results, primarily due to low fractions of fetal-derived cfDNA in maternal plasma, posing challenges for affected families...
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摘要:
Transcription factors drive gene expression dysregulation in cancer. However, non-canonical oncogenic mechanisms of these factors are unclear. Utilizing function-centric proteomics to discover RNA-dependent protein-protein interactions, we uncovered an unexpected interaction between transcription factor oncogene SOX2 and methionine cycle enzyme AHCY. Immunofluorescence and CUT&RUN revealed...