Cellular water potential sensing via biomolecular condensation
Abstract
Water molecules, as solvents for biomolecules, are essential to cells. The water potential of the cell decreases under water-deficient conditions, yet how cells sense changes in water potential remains unknown. Here, we identify a sterile alpha motif (SAM)-containing protein, SAM8, that undergoes water potential-dependent condensation both in vivo and in vitro and is crucial for hyperosmotic stress tolerance and seed germination. We employ biophysical techniques, in vitro reconstitution, and bioimaging to demonstrate that SAM8 is strongly hydrated under normal water conditions, preventing its macroscopic condensation. A negatively charged patch determines SAM8 hydration by creating an electric field and micropolar environment. Water-deficient conditions weaken this hydration, thereby activating SAM8 condensation by reprogramming hydrogen-bond, electrostatic, and hydrophobic interactions. Furthermore, we demonstrate that SAM8 condensates selectively sequester RNA export factors, leading to nuclear retention of mRNAs and translational reprogramming under hyperosmotic stress. Our findings reveal a mechanism by which plant cells directly sense and respond to water status, shedding light on how they adapt to water-deficit conditions.
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