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Elucidating the potassium-binding site and ion-coupled transport mechanism of the serotonin transporter

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Serotonin transporter; 5-HT

摘要

The serotonin transporter (SERT) is responsible for the reuptake of the neurotransmitter serotonin (5-HT), utilizing the Na⁺ gradient across the cell membrane to drive substrate transport. It is proposed that 5-HT is cotransported with one Na⁺ and one Cl⁻, while a potassium ion (K⁺) is transported in the opposite direction. Despite recent structural advances in SERT, the precise intracellular K⁺ binding site still requires experimental structural confirmation. In this study, we capture two previously unreported states of SERT: an inward-open conformation bound with one K⁺ ion and one Cl⁻ ion, and an outward-open conformation free of any ions. These findings provide comprehensive insights into the ion-coupled transport cycle of SERT.

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已发布

2026-04-19

如何引用

Tan, J., Xiao, Y., Kong, F., Zhao, T., Yuan, Y., & Yan, C. (2026). Elucidating the potassium-binding site and ion-coupled transport mechanism of the serotonin transporter. 浪淘沙预印本平台. https://doi.org/10.65215/LTSpreprints.2026.04.19.000191

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