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制备用于冷冻电镜的石墨烯夹层样品

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作者

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关键词
Cryo-EM; Graphene sandwich; Specimen preparation

摘要

低温电子显微镜(cryo-EM)可通过单颗粒分析(SPA)实现大分子近原子分辨率的高分辨率结构测定。然而,空气-水界面(AWD)效应、电子束诱导带电效应和颗粒运动限制了该技术的全部潜力。石墨烯夹层法通过将样品封装在两层石墨烯之间来解决这些挑战,从而提高低温电子显微镜数据的质量。该方法也拓展了石墨烯在电子显微镜中的应用前景。为了简化该方法的实施,本方案详细介绍了制备石墨烯夹层样品和采集相应低温电子显微镜数据的步骤。

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已发布

2026-05-07

如何引用

Liu, B., Xu, J., Gao, X., Zheng, L., Liu, N., Peng, H., & Wang, H.-W. (2026). 制备用于冷冻电镜的石墨烯夹层样品. 浪淘沙预印本平台. https://doi.org/10.65215/LTSpreprints.2026.05.06.000228

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