Preprint / Version 1

Establishment of a Dual-Reporter Minigenome System for Respiratory Syncytial Virus

This article is a preprint and has not been certified by peer review.

Authors

Categories
Immunology & Microbiology
Keywords
Respiratory Syncytial Virus; Minigenome; Antiviral

Abstract

Respiratory syncytial virus (RSV) poses a significant global health challenge, particularly affecting infants, the elderly, and immune compromised individuals. Despite recent progress in the development of vaccines and monoclonal antibodies, effective antiviral therapies remain limited. To advance the discovery of antiviral drugs, we have developed a dual-reporter RSV minigenome system, providing a safe and robust platform for antiviral evaluation. This system incorporates NanoLuc luciferase and superfolder GFP (sfGFP) linked by a self-cleaving P2A peptide, allowing for simultaneous detection of orthogonal signals. Validation with L polymerase inhibitors confirmed the system's reliability for screening small-molecule inhibitors. The linear correlation observed between reporter signals enhances the assay's reliability for antiviral assessment. This dual-reporter minigenome system advances targeted therapeutic strategies against RSV.

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DOI:

https://doi.org/10.65215/LTSpreprints.2026.01.15.000084

Submission ID:

84

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Posted

2026-01-17

How to Cite

Pan, L., Xu, Y., Ma, Y., Zhang, J., & Wu, C. (2026). Establishment of a Dual-Reporter Minigenome System for Respiratory Syncytial Virus. LangTaoSha Preprint Server. https://doi.org/10.65215/LTSpreprints.2026.01.15.000084

Declaration of Competing Interests

The authors declare no competing interests to disclose.

Copyright

The copyright holder for this preprint is the author/funder.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.