Preprint / Version 2

Quantification of Glycan in Glycoproteins via AUCAgent-enhanced Analytical Ultracentrifugation

This article is a preprint and has not been certified by peer review.

Authors

    Xiaojuan Yu,  
    Xiaojuan Yu
    • National Institutes for Food and Drug Control
    Zhaoxing Wang,  
    Zhaoxing Wang
    Chengshi Zeng,   Ruifeng Zhang,  
    Ruifeng Zhang
    Qing Chang,  
    Qing Chang
    Wendan Chu,  
    Wendan Chu
    Qinghua Ma,  
    Qinghua Ma
    • Beijing Zhifei Lvzhu Biopharmaceutical Co., Ltd
    Ke Ma,  
    Ke Ma
    • Beijing Zhifei Lvzhu Biopharmaceutical Co., Ltd
    Lan Wang,  
    Lan Wang
    • National Institutes for Food and Drug Control
    Chuanfei Yu,  
    Chuanfei Yu
    Wenqi Li
    Wenqi Li
Categories
Biochemistry & Biophysics
Keywords
Glycoprotein; Quantification; Analytical ultracentrifugation; AUCAgent

Abstract

As essential biomolecules composed of proteins and carbohydrate moieties, glycoproteins play pivotal roles in numerous biological processes. The glycosylation level plays a crucial role in determining the functionality of glycoproteins. Therefore, the precise quantification of glycan components in proteins holds significant importance for research on and development of polysaccharide-protein-conjugated vaccines. In this study, a novel glycan quantification approach was developed, leveraging analytical ultracentrifugation (AUC) technology that synergistically utilizes ultraviolet wavelength absorption and interference data to directly determine glycan mass fractions in glycoproteins. This methodology expands the analytical framework for glycoproteins while retaining the intrinsic advantages of AUC, enabling analysis in native states with demonstrated robustness. The approach was implemented in our proprietary AUC data analysis software called AUCAgent.

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DOI:

https://doi.org/10.65215/j1ch6d86

Submission ID:

54

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Posted

2025-12-14

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How to Cite

Yu, X., Wang, Z., Zeng, C., Zhang, R., Chang, Q., Chu, W., Ma, Q., Ma, K., Wang, L., Yu, C., & Li, W. (2025). Quantification of Glycan in Glycoproteins via AUCAgent-enhanced Analytical Ultracentrifugation. LangTaoSha Preprint Server. https://doi.org/10.65215/j1ch6d86

Declaration of Competing Interests

The authors declare no competing interests to disclose.

Copyright

The copyright holder for this preprint is the author/funder.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.