Preprint / Version 1

Single-Cell Multi-omics Decodes the Aggrephagy Landscape, Unveiling TUBA4A as a Novel Oncogene and Immunotherapeutic Target in ccRCC

This article is a preprint and has not been certified by peer review.

Authors

    Kun Cui,   Qiuxin Duan,   Zhe Xu,   Wenjing Liu,   Yueli Ni,   Yongping Li,   Lu Yuan,   Zhuoran Teng,   Yurong Dong,   Ziyuan Bai,   Asif Shahzad,   Jianshan Zhang,   Yu Dou,   Jiaojiao Xia,   Hanyue Shao,   Zhe Yang,   Qiao Zhang
    Qiao Zhang
Categories
Biochemistry & Biophysics Molecular Biology
Keywords
ccRCC; aggrephagy; tumor microenvironment; prognosis

Abstract

Clear cell renal cell carcinoma (ccRCC) is an aggressive malignancy with a complex and immunosuppressive tumor microenvironment (TME). While aggrephagy, a selective autophagy process, has been linked to cancer, its role in ccRCC remains unclear. Through integrated multi-omics analysis of single-cell and bulk sequencing data, we constructed the first aggrephagy activity atlas in ccRCC TME, revealing subtype-specific programs linked to immune suppression and metabolic reprogramming. High aggrephagy activity correlated with poor prognosis and immunotherapy resistance, particularly in macrophages and T cells. Among key prognostic genes, we identified TUBA4A as a novel player in ccRCC. Functional validation showed that TUBA4A enhances autophagy and promotes tumor proliferation, migration, and invasion. This study establishes aggrephagy and TUBA4A as key regulators of ccRCC progression and potential therapeutic targets. 

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DOI:

https://doi.org/10.65215/1wp90106

Submission ID:

3

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Posted

2025-11-18

How to Cite

Cui, K., Duan, Q., Xu, Z., Liu, W., Ni, Y., Li, Y., Yuan, L., Teng, Z., Dong, Y., Bai, Z., Shahzad, A., Zhang, J., Dou, Y., Xia, J., Shao, H., Yang, Z., & Zhang, Q. (2025). Single-Cell Multi-omics Decodes the Aggrephagy Landscape, Unveiling TUBA4A as a Novel Oncogene and Immunotherapeutic Target in ccRCC. LangTaoSha Preprint Server. https://doi.org/10.65215/1wp90106

Declaration of Competing Interests

The authors declare no competing interests to disclose.

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The copyright holder for this preprint is the author/funder.

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