生物化学与生物物理学
所有项目
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摘要:
Human M-channels, primarily assembled by heteromeric KCNQ2/KCNQ3 subunits, are critical regulators of neuronal excitability, and loss-of-function mutations in either subunit are linked to epileptic disorders. Yet, the molecular mechanisms underlying heteromeric assembly, gating, and pharmacological modulation have remained largely elusive. Here, we present high-resolution...
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摘要:
The M-channel, a heterotetrameric voltage-gated potassium channel formed by KCNQ2 and KCNQ3 subunits, critically regulates neuronal excitability, with dysfunction linked to epilepsy and developmental encephalopathies. Despite its physiological importance, structural mechanisms governing its unique heteromeric assembly...
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摘要:
As essential biomolecules composed of proteins and carbohydrate moieties, glycoproteins play pivotal roles in numerous biological processes. The glycosylation level plays a crucial role in determining the functionality of glycoproteins. Therefore, the precise quantification of glycan components in proteins holds significant importance for research on and...
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摘要:
Biomolecular condensates formed through liquid–liquid phase separation (LLPS) organize essential cellular functions as membraneless organelles. Understanding their internal atomic-level chemistry is critical, but inaccessible to classical nuclear magnetic resonance (NMR) due to their picoliter volumes. While emerging quantum sensing has extended NMR to this scale, applying this capability to...
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摘要:
Identification of individual cells within heterogeneous populations is essential for biomedical research and clinical diagnostics. Conventional labeling-based sorting methods, such as fluorescence-activated cell sorting and magnetic-activated cell sorting, enable precise sorting when reliable markers are available. However, their applicability is limited in cells lacking defined markers or...
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摘要:
Almost all the reported cryo-EM structures of eukaryotic voltage-gated sodium (Nav) channels, including those of human Nav1.1-Nav1.8, represent various inactivated states that are characteristic of a non-conductive pore domain (PD) surrounded by...
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摘要:
The landscape of catalytic RNA in complex eukaryotes remains poorly charted. Although self- cleaving ribozymes are widespread in microbial and viral genomes, their existence and functional roles in humans remain limited. Here, we introduce a generalizable, genome-wide discovery platform that integrates...
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摘要:
With CryoSeek, a structure-first paradigm for discovery, we have determined high resolution 3D structures of a number of glycofibrils, in which well-ordered glycans either form a thick shell coating various protein cores or constitute the entire fibril. To improve the throughput of CryoSeek, we hereby report two methods. The recursive bisection clustering...
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摘要:
The functional diversification of biosynthetic enzymes underlies the chemical richness of natural products, yet how primary metabolic enzymes...
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摘要:
Clear cell renal cell carcinoma (ccRCC) is an aggressive malignancy with a complex and immunosuppressive tumor microenvironment (TME). While aggrephagy, a selective autophagy process, has been linked to cancer, its role in ccRCC remains unclear. Through integrated multi-omics analysis of single-cell and bulk sequencing data, we constructed the first aggrephagy activity atlas in ccRCC TME,...