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• Cellular synthesis of ubiquitin-based probes for spatially resolved deubiquitinase profiling

  Authors: Jinghao Chen, Xinyu Zhang, Yang Huang, Gang Li, Yu-Hsuan Tsai

  Abstract:

  Deubiquitinases (DUBs) orchestrate ubiquitin homeostasis, yet their compartment-specific activities remain underexplored. We developed a genetically encoded

  Keywords: Activity-based protein profiling; Deubiquitinase (DUB); Proximity-induced chemistry; Sortase A (SrtA); Ubiquitination

  Timeline: First Posted 2025-11-30 | Last Updated 2025-11-30

  Metrics: Views: 647  |  Downloads: 336  |  Favorites: 0

• Disulfide-directed multicyclic peptides for chimeric antigen receptors targeting solid tumors

  Authors: Xiaoting Meng, Keke Fu, Yawei Liu, Yuan Liu, Joe Zhang, Xi Kang, Chuanliu Wu, Yu-Hsuan Tsai

  Abstract:

  The clinical application of chimeric antigen receptor (CAR) T cell therapy in solid tumors remains limited due to significant safety concerns, particularly “on-target, off-tumor” toxicity and cytokine release syndrome (CRS). Here, we describe a class of CARs that employ disulfide-directed multicyclic peptides (DDMPs) as compact antigen-recognition domains targeting the tumor-associated...

  Keywords: CAR T; cyclic peptide; on-target, off-tumor toxicity; cytokine release syndrome; chimeric antigen receptor; disulfide-directed multicyclic peptide (DDMP)

  Timeline: First Posted 2025-11-25 | Last Updated 2025-11-25

  Metrics: Views: 459  |  Downloads: 282  |  Favorites: 0

• Mucinase-engineered cell membrane nanovesicles degrade the glycocalyx shield to potentiate antitumor immunity

  Authors: Xiaorui Geng, Silan Liu, Yuanwei Pan, Yun Ge, Lang Rao

  Abstract:

  The tumor glycocalyx forms a protective shield that masks checkpoint proteins and compromises the efficacy of immunotherapies. While the bacterial protease StcE can degrade this barrier by cleaving O-glycosylated mucin domains, its therapeutic potential is hindered by off-target toxicity and high immunogenicity. To overcome these limitations, we developed a biomimetic platform of cell...

  Keywords: Glycoengineering; Nanobiotechnology; Cellular vesicles; Drug delivery; Cancer immunotherapy

  Timeline: First Posted 2025-11-19 | Last Updated 2025-11-19

  Metrics: Views: 659  |  Downloads: 462  |  Favorites: 3