Bioengineering & Biotechnology
All Items
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Abstract:
Autologous chimeric antigen receptor T (CAR-T) cell therapy has demonstrated therapeutic effectiveness in hematologic malignancies and autoimmune diseases. However, the manufacturing complexity and the required lymphodepletion hindered its wide clinical application. Engineering human T cells in vivo holds promise to conquer these limitations but requires effective T cell-targeted CAR delivery...
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Abstract:
S-PET is a compact, high sensitivity and high resolution small animal PET prototype developed at Shenzhen Bay Laboratory (SZBL). The S-PET system employs a phoswich depth of interaction (DOI) detector design, which also allows identification of the large majority of the cross layer crystal scatter (CLCS) events. This work evaluates its performance characteristics following the National Electrical...
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Abstract:
High-throughput detection of gene expression changes enables novel biological discovery. However, current methods suffer limitations. Methods coupled with RNA-sequencing involve higher costs and technological barriers while gene reporter assays only account for promoter activity. Here, we developed a high-throughput, amplification-free, and accessible method, Cellular Fluorescence Anisotropy of...
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Abstract:
Atrial fibrillation (AF) is unexpectedly prevalent in Brugada syndrome (BrS), yet the mechanisms linking SCN5A loss-of-function to atrial instability remain elusive. Here, we combined patient-specific induced pluripotent stem cell-derived atrial cardiomyocytes with label-free high-density microelectrode array (HD-MEA) mapping. We show that SCN5A haploinsufficiency creates an arrhythmogenic...
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Abstract:
Current fluorescent reporters for assessing activity of CRISPR-Cas system, such as Stoplight or split-GFP based on frameshift activation, often suffer from limitations including large vector size, low viral packaging efficiency, and insensitivity to in-frame insertions or deletions. Although specialized reporters for base editing (e.g., BEAR) and prime editing (e.g., PEAR and fluoPEER) have been...
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Abstract:
Ultrasound mediated microbubble cavitation holds great potential for non-invasive and targeted drug delivery. However, the interplay between acoustic parameters, bubble dynamics, and resulting cellular responses remains unclear, hindering the safety improvement and optimization of the technique. This study examined the effects of ultrasound pulse sequences on microbubble dynamics and bioeffects...
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Abstract: Objective: Noninvasive prenatal testing (NIPT) based on cell-free DNA (cfDNA) analysis is widely used for detecting fetal aneuploidies globally, such as Trisomy 21 (T21) testing. Despite its high sensitivity, current NIPT methods have a non-negligible rate of false-negative results, primarily due to low fractions of fetal-derived cfDNA in maternal plasma, posing challenges for affected families...
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Abstract:
To achieve a PET system that simultaneously offers high sensitivity, high spatial resolution, improved signal to noise ratio (SNR), and depth of interaction (DOI) measurement, we propose and implement a high DOI resolution PET detector based on a 1.5 × 1.5 × 20 mm3 BGO crystal array. In addition, we introduce a vertical line-source irradiation DOI calibration method, which enables simultaneous...