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• Open-state structure of veratridine-activated human Na_v1.7 reveals the molecular choreography of fast inactivation

  Authors: XIAO FAN, Jiaofeng Chen, Lingfeng Xue, Huan Wang, Tong Wu, Xiaoshuang Huang, Fangzhou Lu, Xueqin Jin, Chen Song, Jian Huang, Nieng Yan (Author)

  Abstract: Almost all the reported cryo-EM structures of eukaryotic voltage-gated sodium (Na v ) channels, including those of human Na v 1.1-Na v 1.8, represent various inactivated states that are characteristic of non-conductive pore domain (PD) and voltage-sensing domains (VSDs) activated to varying degrees. To capture an open-state Na v structure, we treated purified human Na v 1.7 with veratridine...

  Keywords: Voltage gated sodium channel , Open-state , Fast inactivation , Veratridine

  Posted: 2025-11-18

  Metrics: Views: 254  |  Downloads: 21  |  Favorites: 2

• Achieving Near-Atomic Resolution in Single-Particle Cryo-EM Using a Single Micrograph

  Authors: Zi Yang, Xiao Fan, Hongwei Wang (Author)

  Abstract: Single-particle cryo-electron microscopy (cryo-EM) typically requires the acquisition and analysis of a large number of micrographs to achieve high-resolution three-dimensional (3D) reconstructions of biological macromolecules. In this study, we demonstrate that when sample quality is sufficiently high, near-atomic resolution 3D density maps can be obtained using particle images from a single...

  Keywords: Single-Particle Cryo-EM , Single-image reconstruction

  Posted: 2025-11-18

  Metrics: Views: 21  |  Downloads: 6  |  Favorites: 2

• Single-Cell Multi-omics Decodes the Aggrephagy Landscape, Unveiling TUBA4A as a Novel Oncogene and Immunotherapeutic Target in ccRCC

  Authors: Kun Cui, Qiuxin Duan, Zhe Xu, Wenjing Liu, Yueli Ni, Yongping Li, Lu Yuan, Zhuoran Teng, Yurong Dong, Ziyuan Bai, Asif Shahzad, Jianshan Zhang, Yu Dou, Jiaojiao Xia, Hanyue Shao, Zhe Yang, Qiao Zhang (Author)

  Abstract: Clear cell renal cell carcinoma (ccRCC) is an aggressive malignancy with a complex and immunosuppressive tumor microenvironment (TME). While aggrephagy, a selective autophagy process, has been linked to cancer, its role in ccRCC remains unclear. Through integrated multi-omics analysis of single-cell and bulk sequencing data, we constructed the first aggrephagy activity atlas in ccRCC TME,...

  Keywords: ccRCC , aggrephagy , tumor microenvironment , prognosis

  Posted: 2025-11-18

  Metrics: Views: 17  |  Downloads: 2  |  Favorites: 0

• Evolutionary repurposing of a metabolic thiolase complex enables antibiotic biosynthesis

  Authors: Ge Liao, Ruolan Sun, Zilin Shen, Zhiteng Luo, Cuiping Pang, Zhuanglin Shen, Anfu Wei, Chengneng Mi, Gengfan Wu, Yong-Xin Li, Kin Kuan HOI, Xiaojing Pan, Xiaoyu Tang (Author)

  Abstract: The functional diversification of biosynthetic enzymes underlies the chemical richness of natural products, yet how primary metabolic enzymes evolve to acquire specialized functions in secondary metabolism remains elusive. Here, we report a tripartite enzyme complex from oral Streptococcus species—comprising 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase (HMGS), acetyl-CoA acetyltransferase...

  Keywords: thiolase; enzyme complex; Friedel-Crafts acylation; biosynthesis; functional evolution

  Metrics: Views: 14  |  Downloads: 2  |  Favorites: 0